Molecular cloning, sequence analysis of mouse integrin ?_4 gene and repairing of its mutation
- VernacularTitle:小鼠整合素?_4基因克隆、序列分析及突变修复
- Author:
Yongming WANG
;
Yan HU
;
Haiqi LI
- Publication Type:Journal Article
- Keywords:
?4 gene;
cDNA clone;
RT-PCR;
mutation repairing
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and analyze the full-length cDNA of mouse integrin ?4, and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of ?4 gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of ?4 cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse ?4 had a length of 3 099 bp, and encoded a product of 1 032 amino acids. There were 12 bases pairs mutation of ?4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse ?4 is cloned successfully.
