Action potential of cardiac pacemaker cells differentiated from mouse mesenchymal stem cells after HCN4 gene modification
- VernacularTitle:HCN4基因修饰大鼠MSCs体外诱导获得的心脏起搏样细胞动作电位检测
- Author:
Zewen WANG
;
Zhiyuan SONG
;
Qing YAO
- Publication Type:Journal Article
- Keywords:
cardiac pacemaker cells;
action potential;
whole cell patch
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potassium currents of the cardiac pacemaking cells induced and differentiated from rat mesenchymal stem cells (MSCs) modified by HCN4 gene. Methods Identified cardiac pacemaking cells were adopted as the experiment group, and the sinoatrial node cells of original infant rat cultured in the same period were regarded as the control group. Whole cell patch was used to measure the action potential of the pacemaking cells and sinoatrial node cells. Results Action potential of automatic depolarization at dilatation was recorded in both the differentiated cardiac pacemaking cells and sinoatrial node cells. There was no significant difference on amplitudes of resting potential, amplitudes and cycle of action potential [(-50?2.8) vs (-55?5.5),(-60?2.5) vs (-65?2.5),(240?57) ms vs (250?60) ms], but the field potential was much lower in cardiac pacemaking cells than the control group[(-30?2.5) vs (-55?5.5),P
