The initial research on the effect of anti-stressed depression of Jiaweisinisan and it's mechanism of N-methyl-D-aspartate receptor channel in hippocampus
- VernacularTitle:加味四逆散抗应激性抑郁效应及其海马NMDA受体通道机制的初步研究
- Author:
Lili WU
;
Can YAN
;
Shengyuan DING
;
Zhiwei XU
;
Chun ZENG
;
Jinxin LAN
- Publication Type:Journal Article
- Keywords:
stress;
depression;
hippocampus;
N-methyl-D-aspartate;
receptor;
Jiaweisinisan
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the anti-stress depression effect of Jiaweisinisan(JWSNS)and it's mechanism of N-methyl-D-aspartate(NMDA)receptor channel in hippocampus.Methods Chronic mild unpredictable stress(CMUS)was adopted to establish the rat depres-sion model.Before and after model establishing,the sucrose consumptions and the weight of rats were detected.TTC dyeing of hippocampus brain slices ex vivo were used to detect the situation of hippocampus neuron damage and the protective effect of serum containing components of JWSNS.The opening probability and the average opening time of NMDA receptor channels were detected by attach on cell patch clamp,the Ca2+ concentrations in single cell of hippocampus neurons were detected by Tillvis ION software.Results CMUS could decrease the sucrose preference and the weights of rats(P
