Effects of emodin on IL-8 secretion and NF-?B activation of HT-29 cells
- VernacularTitle:大黄素对HT-29细胞IL-8分泌及NF-?B活化的影响
- Author:
Qing WANG
;
Ting ZHOU
;
Lian ZHOU
;
Yan DONG
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
emodin;
HT-29 cell;
IL-8;
NF-?B
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of emodin on IL-8 secretion and NF-?B activation of HT-29 cells,and explore the molecular mechanism of emodin.Methods The cytotoxicity of emodin was assessed by WST;NF-?B activation was detected with co-focal microscopy by immunofluorescence;the production of IL-8 was investigated by ELISA.Results Emodin with the concentration of 10~80 ?mol?L-1 could decrease the mass production of IL-8 Secretion of HT-29 cells stimulated by IFN-?+LPS in a dose-dependent manner.Emodin with various concentrations could inhibit NF-?B activation dose-dependently.Conclusions Emodin inhibited IL-8 secretion and NF-?B activation of HT-29 cells stimulated by IFN-?+LPS.
