Effect of Taurine on the cell cycle of cardiac fibroblast and its mechanisms
- VernacularTitle:牛磺酸对心肌成纤维细胞细胞周期的影响及其机制的研究
- Author:
Yanchun WANG
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
taurine;
cardiac fibroblast;
cell cycle regulatory protein;
p27
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of taurine(Tau)on the cardiac fibroblast(CFb)cell cycle and its regulatory protein expression,and to explore the underlying mechanism of Tau-inhibiting the proliferation of CFb.Methods The proliferation of cultured neonatal rat CFb was induced by Angiotensin Ⅱ and detected by the thiazole blue(MTT)colorimetric assay.Hydroxyproline measurement was carried out to detect the collagen quantification.The expression of cell cycle regulatory protein cyclin D and p27 were determined by the combination of immunocytochemical staining and image analysis software.Cell cycle and p27 were assessed via flow cytometry.Results CFb proliferation and hydroxyproline content in culture medium were markedly inhibited when CFb were treated with taurine at concentrations of 40,80 and 160 mmol?L-1 for 24 hours(P
