Establishment of the oxidative damage model in brain of PD rats induced by L-DOPA with microdialysis technique
- VernacularTitle:应用脑微透析技术建立L-DOPA引起的PD大鼠脑氧化损伤的模型
- Author:
Danqiao WANG
;
Wei WANG
;
Fuchun JING
;
Jinning ZHAO
- Publication Type:Journal Article
- Keywords:
levodopa;
microdialysis in brain;
hydroxyl radical;
6-hydroxyl dopamine;
oxidative damage
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the model of oxidative damage in brain of Parkinsons disease(PD)rats induced by levodopa(L-DOPA)with microdialysis technique.Methods PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine(6-OHDA)and were perfused in brain with L-DOPA by using microdialysis technique.Salicylic acid can capture hydroxyl radicals in brain,then yield 2,3-dihydroxy benzyl acid(2,3-DHBA)and 2,5-dihydroxy benzyl acid(2,5-DHBA).Extracelluler dopamine(DA)and its metabolites,2,3-DHBA and 2,5-DHBA in striatum of rats were measured by HPLC-ED before and after L-DOPA treatment.Results Both 2,3-DHBA and 2,5-DHBA in model group were significantly higher than those in control group at 6 and 7 time points respectively(P
