Effects of anti-HER-2 chimeric antibody chA21 on proliferation and apoptosis of SKBR3 cells
- VernacularTitle:抗HER-2嵌合抗体chA21对SKBR3细胞的增殖抑制及凋亡诱导作用
- Author:
Hua XUE
;
Qiang WU
;
Xiangyang HU
;
Xiaoguang LING
;
Feng YANG
;
Liansheng CHENG
;
Jing LIU
- Publication Type:Journal Article
- Keywords:
HER-2;
chimeric antibody;
chA 21;
breast cancer;
SKBR3;
proliferation;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of anti-HER-2 chimeric antibody chA 21 on proliferation and apoptosis of SKBR3 cells.Methods MTT colorometric assay,HE staining,transmission electron microscopy,flow cytometry,and TUNEL were used to study the proliferation inhibition and apoptosis induction of SKBR3 cells by chA 21 in vitro.Results Proliferative inhibition rates and apoptotic index of SKBR3 cells were increased in a dose and time dependent manner after exposure to chA21(0.2～5.4 mg?L~(-1)).Conclusion chA 21 could remarkably inhibit proliferation of SKBR3 cells in vitro and apoptosis induction may be one of its main mechanisms.
