Effect of peptide from agkistrodon acutus snake venom on adhesion and proliferation of human ovarian carcimona A2780 cell line
- VernacularTitle:尖吻蝮蛇毒小分子多肽对人卵巢癌A2780细胞增殖和黏附的影响
- Author:
Danqing LEI
;
Yingxin LI
;
Xianguo ZHOU
- Publication Type:Journal Article
- Keywords:
Agkistrodon acutus venom;
ovarian carcimona;
proliferation;
adhesion;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibitory effect of peptide from Agkistrodon Acutus snake venom(fraction K)on the growth of human ovarian carcimona A2780 cell line and its mechanism.Methods The A2780 cells were treated with fraction K at different concentrations.The proliferation of A2780 cells was assayed with MTT colorimetric method.The histologi-cal and ultras-tructural changes of cell were scored using light and electron microseopy.The apoptosis of A2780 cells induced by fraction K was studied by fluorescent staining.Crystal violet staining and MTT assay were employed to determine the effect of fraction K on A2780 cells that they had been adhered to fibronectin.Result Proliferation of A2780 cells was inhibited significantly by fraction K in a dose-and time-dependant manner.Under electron-microseop and fluorescence microscope,some A2780 cells underwent a typical apoptosis and morphology changes after 24 hours incubation with fraction K.Fraction K could inhibit the adhesion of A2780 cells to fibronectin in a dose-dependent manner.Conclusion fraction K can inhibit proliferation of A2780 cells,which may be related to the mechanism of inducing cellular apoptosis and cellular adhesion.
