PTEN gene expression in mouse endometria increases during embryo implantation
- VernacularTitle:PTEN基因在小鼠胚胎着床过程中作用的实验研究
- Author:
Xiaoling CHEN
;
Rong YANG
;
Yongcun JIA
;
Xiaoyun LIU
;
Shali WEI
- Publication Type:Journal Article
- Keywords:
PTEN;
endometrium;
implantation;
real-time fluorescence quantitative PCR;
intrauterine injection
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.Methods Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice(day 1,day 3,day 4,day 5 and day 7),with 20 mice sacrificed at each fixed day.Out of another 20 3-day pregnant mice,ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.Results The PTEN mRNA/?-actin mRNA in pregnant mice was higher than that of nonpregnant mice,gradually hoisted as days passed by,and reached the highest at pregnant day 5.The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.
