Experimental Study on Epithelial-to-mesenchymal Transition Induced by Interleukin-1?
- VernacularTitle:白介素-1?诱导肾小管上皮细胞向间充质细胞转化的实验研究
- Author:
Ruchun YANG
;
Ying LU
;
Xiaoling ZHU
;
Yongjun WANG
- Publication Type:Journal Article
- Keywords:
Interleukin-1?;
Kidney tubules;
Phenotype;
Epithelial-mesenchymaltransition
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the inflammatory factors to increase the reanl inferstitial fibrosis by stndying on epithelial-to-mesenchymal transition induced by IL-1?.Methods Mice primary tubular epithelial cells were Cultured in vitro,then stimulated by IL-1? for 3,4,5 days respectively,from which the protein was extracted and the protein expression of ?-SMA and Vimentin was detected with western bloHing;while the cells were stimnlated by IL-1? for 12hs,36hs and 60hs respectively,from which then total RNA was extracted and the mRNA expression of ?-SMA and Vimentin was detected by ranscription-polymerase chain reaction analyses(RT-PCR).those without IL-1?stimulating were regarded as control group.Results The protein levels of ?-SMA,Vimentin in tubular epithelial cells stimulated with IL-1?for 3 days or 4 days were significantly higher than that in normal group cells.Those stimulated for 5 days,the protein levels of ?-SMA,Vimentin in tubular epithelial cells increased very high.The mRNA expression of ?-SMA and Vimentin increased significantly in 12hs in tubular epithelial cells after IL-1?stimulating as compared with normal group cells and presented time-depend.Conclusions IL-1? plays the role of promoting renal interstitial fibrosis by inducing the transdifferentiation of renal tubular epithelial cells and reducing the proliferation cells probably.
