MRI of the transplanted endothelial progenitor cells for prevent atherosclerotic plaque formation
- VernacularTitle:血管内皮祖细胞移植防治动脉粥样硬化形成的MRI实验研究
- Author:
Zhanlong MA
;
Gaojun TENG
;
Xiaoli MAI
;
Jun CHEN
;
Junhui SUN
;
Hongying ZHANG
;
Hui YU
;
Guozhao LI
- Publication Type:Journal Article
- Keywords:
Endothelium,vascular;
Endothelial cells;
Arteriosclerosis;
Cell transplantation;
Rabbits;
Magnetic resonance imaging
- From:
Chinese Journal of Radiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the 1.5 T magnetic resonance imaging system to depict and track in vivo of magnetically labeled endothelial progenitor cells(EPCs),and to study the possibility for preventing the atherosclerotic plaque formation in New Zealand rabbit model of carotid arterial injury after transplantation.Methods New Zealand rabbit EPCs were isolated,confirmed,expanded and then incubated with home synthesized Fe_2O_3-PLL,Prussian blue stain was performed for showing intracellular irons.The model of carotid arterial injury was performed by 2.5F balloons,the group A of 8 rabbits received magnetically labeled EPCs,group B of 3 rabbits received fluorescent-labeled EPCs and the group C of 5 rabbits were given same volume saline injection after endothelial injury of the carotid artery.MR imaging and histology were performed and compared 4 days later for randomly chosen three rabbit,each from one of the three group;all the other rabbits were fed with high lipid diet and examed using MR imaging and histology after 15 weeks.Results Epcs labeling efficiency was more than 95% by Prussian blue stain, 4 days after transplantation of EPCs,only in group A,the injured endothelium of carotid artery had signal intensity loss in T_2 * WI,which were correlated well with the area where the most Prussian blue staining positive cells were found in histopathology analyses.The rabbits of group A and B which received EPCs transplantation exhibited fewer plaques formation than those of the group C(P
