Tissue-Specific Regulation of Angiotensinogen and Angiotensin II Receptor Genes in Two-Kidney, One Clip Hypertensive Rats.
- Author:
Jong Un LEE
1
;
Mi Ra AN
;
Sang Chae NAM
;
Won Jae KIM
Author Information
1. Department of Physiology, Chonnam University Medical School, Korea.
- Publication Type:Original Article
- Keywords:
Renin;
Angiotensinogen;
Angiotensin II receptor;
Northern blot analysis;
Two-kidney;
One clip hypertension
- MeSH:
Angiotensin II*;
Angiotensinogen*;
Angiotensins*;
Animals;
Blotting, Northern;
Hand;
Hypertension;
Kidney;
Liver;
Rats*;
Receptors, Angiotensin*;
Renin
- From:Korean Journal of Nephrology
1999;18(4):523-529
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The present study was aimed at investigating the molecular regulation of the renin- angiotensin system (RAS) in two-kidney, one clip (2K1C) hypertension. The expression of renin, angiotensinogen and angiotensin II receptor genes was determined by Northern blot analysis in rats made 2K1C hypertensive for 2 or 4 weeks. The expression of renin gene was increased in the clipped kidney and decreased in the contralateral non-clipped kidney at weeks 2 and 4. The expression of angiotensinogen gene was not significantly altered at week 2, but increased at week 4 in the clipped kidney. However, it was not significantly altered in the contralateral kidney either at week 2 or 4. Nor was the expression of angiotensinogen gene significantly altered in the liver either at week 2 or 4. On the other hand, the expression of angiotensin II receptor gene was decreased at week 2, and increased at week 4 in the clipped kidney, whereas it was not significantly changed in the contralateral kidney either at week 2 or 4. In the liver, the expression of angiotensin II receptor gene was not significantly altered at week 2, but decreased at week 4. These results suggest that the components of RAS are transcriptionally regulated in 2K1C hypertension in a manner dependent on tissues and duration of hypertension.
