Clinical study of the application of phage amplified biologically assay in the detection of mycobacterium tuberculosis in sputum
- VernacularTitle:噬菌体生物扩增法检测痰结核分枝杆菌的临床研究
- Author:
Xiuli YU
;
Qiuyue WANG
;
Yanling LI
- Publication Type:Journal Article
- Keywords:
Mycobacterium,tuberculosis;
Mycobacteriophages
- From:
Chinese Journal of Practical Internal Medicine
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the application of the phage amplified biologically assay (PhaB) in the detection of the mycobacterium tuberculosis (TB) in sputum.Methods To determine the TB patients in the 53 suspected patients we detected 53 sputum samples by PhaB method,BACTEC MGIT960 rapid culture method,and conventional methods (direct auramine smear microscopy and concentrated auramine smear microscopy) individually.Results TB positive samples detected by PhaB,BACTEC MGIT960 rapid culture,direct auramine smear microscopy and concentrated auramine smear microscopy were 24,22,11 and 17 respectively.Thus the sensitivity,specificity,positive predictive value and negative predictive value were 86.4%,83.9%,79.2% and 89.7%,when the PhaB is combined with concentrated auramine smear microscopy,the sensitivity,specificity,positive predictive value and negative predictive value were increased up to 90.9%,83.9%,80.8% and 92.9%.Conclusion PhaB method is a simple and rapid method to detect the Mycobacterium Tuberculosis in sputum with a relatively higher sensitivity and specificity than the conventional methods.When combined with concentrated auramine smear microscopy,PhaB method is even more sensitive.Thus the PhaB method is accessible and applicable in the clinical use of the TB detection.
