Detection of anti-histoplasma antibody in sera of immunized mice by dot immunogold filtration assay
- VernacularTitle:斑点免疫金渗滤法检测抗荚膜组织胞浆菌抗体的研究
- Author:
Pu WANG
;
Baowen CHEN
;
Miao XU
;
Guozhi WANG
- Publication Type:Journal Article
- Keywords:
Histoplasma;
dot immunogold filtration assay;
histoplasma antibody
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study a new and rapid method for detection of anti-histoplasma antibody by dot immunogold filtration assay (DIGFA). Methods DIGFA was developed by coating purified protein derivative of histoplasmin (P-HTPM) on nitrocellulose membrane as membrane antigen and labeling SPA with colloidal gold. Anti-histoplasma antibodies in sera from mice immunized with Histoplasma were detected by DIGFA. Results All immunizedsera with Histoplasma were positive by DIGFA as well as the results detected by ELISA. The sera immunized respectively with Monilia and Penicillium marneffei were negtive by DIGFA while 1 of 8 immunizedsera with Blastomyces dermatitidis and 2 of 9 immunizedsera with Paracoccidioides brasiliensis were found to be positive. Conclusion DIGFA was a valuable and rapid method to detect histoplasmaantibody in serum.
