The probable pathway of low concentration of ouabain on intracellular calcium elevation in guinea ventricular mycytes
- VernacularTitle:低浓度的哇巴因引起豚鼠心室肌细胞内钙增高的可能途径
- Author:
Chen XIONG
;
Yanzhao WU
;
Huicai GUO
;
Yongli WANG
- Publication Type:Journal Article
- Keywords:
Na,K-ATPase;
ventricular myocytes;
confocal microscopy;
intracellular carcium
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effects of low concentration ouabain(OUA)on intracellular calcium concentration ([Ca2+]i) were investigated in guinea pig ventricular myocytes.Methods The guinea-pig ventricular myocytes were obtained by enzymatic dissociation technique, then were incubated with Fluo3-AM. The Fluo3-AM fluorescent signal was detected with confocal laser scanning microscopy. The change of [Ca2+]i was represented by the percentage of fluorescence intensity change [(FI-FI0)/FI0,%] (FI: fluorescent intensity after addimg OUA, FI0: control). Results In normal Tyrode,s solution and Ca2+-free Tyrode′s solution.OUA (1?10-9~1?10-6 mol?L-1)elevated [Ca2+]i in a concentration-dependent manner, in normal Tyrode′s solution by 16.7?6.8(P0.05).Genistein (GST) (1、10、50、100 ?mol?L-1)abolished the OUA-induced increases in[Ca2+]i in a concentration-dependent manner by 17.5?3.1、14.2?8.9、0.8?7.6(P
