The role of tumor necrosis factor related apoptosis inducing ligand and Polypeptide from Chlamys farreri in UVA-induced apoptosis in HaCaT cells
- VernacularTitle:肿瘤坏死因子相关凋亡诱导配体及扇贝多肽在UVA诱导HaCaT细胞凋亡中的作用
- Author:
Zhenli WANG
;
Jinlian LI
;
Chunbo WANG
- Publication Type:Journal Article
- Keywords:
UVA;
Polypeptide from Chlamys farreri(PCF);
HaCaT;
tumour necrosis factor related apoptosis inducing ligand(TRAIL);
caspase-8;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the impact of UVA on expression of tumour necrosis factor related apoptosis inducing ligand (TRAIL) and study the role of TRAIL in UVA-induced apoptosis of HaCaT cells as well as the influence of Polypeptide from Chlamys farreri(PCF)on TRAIL apoptotic pathway induced by UVA.Methods Cells were divided into five groups: control group, UVA model group,UVA+5.69 mmol?L-1 PCF group, UVA+2.84 mmol?L-1 PCF group, UVA+1.42 mmol?L-1 PCF group.Expression level of TRAIL mRNA was assayed by Real-Time PCR.Western blot analysis was used to determine the protein level of TRAIL and caspase-8 activation. The effect of TRAIL neutralization antibody on UVA-induced apoptosis was also investigated.Results TRAIL mRNA and protein levels increased after 8 J?cm-2 UVA radiation and the discrepancy was significant compared with control group(P
