A high throughput screening model for P-gp functional inhibitors
- VernacularTitle:P-gp蛋白功能抑制剂高通量筛选模型的建立与应用
- Author:
Tingyuan LANG
;
Jufang YAN
;
Changhua HU
- Publication Type:Journal Article
- Keywords:
P-glycoprotein;
inhibitors;
high throughput screening;
traditional Chinese medicine sample
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a cell model for high throughput screening and search for novel inhibitors of P-gp.Methods A fluorescent cell-based assay was developed for application in high throughput screening.A library of 480 traditional Chinese medicine samples was screened. Results The value of Z′ was 0.72. Screening of a library of 480 traditional Chinese medicine identified 2 positive samples.Conclusions This HTS model for screening P-gp inhibitors was stable and sensitive.The 2 positive samples had good P-gp inhibitory activities.
