Optimization of taxol extraction from Taxus baccata L.
- VernacularTitle:紫杉醇提取工艺的优化试验研究
- Author:
Xian YANG
;
Shuiping YANG
;
Xue ZHANG
;
Jie TAN
- Publication Type:Journal Article
- Keywords:
Taxus baccatal L.;
taxol;
HPLC;
orthogonal test
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize and establish the extractive technology of taxol from Taxus baccatal L.. Methods HPLC was applied to determine the content of taxol. The orthogonal test was adopted to examine the effects of the 4 factors consisting of ethanol concentration,ethanol volume,extractive times and extractive time. Results Ethanol concentration and ethanol volume were essential factors influencing the extraction of taxol. High concentration and amount of ethanol facilitated the extraction. Extractive times and extractive time were also important. By single-factorial effect,2 h and 2 times in each extraction of taxol were the best. Conclusion The optimum extractive condition is T9 (A3B3C2D1) and T8 (A3B2C1D3).
