Expression of NF-?B in the brain and lung and its role in acute lung injury following intracerebral hemorrhage of rats
- VernacularTitle:大鼠脑出血后脑肺组织NF-?B的表达及其在急性肺损伤中的作用
- Author:
Xianwei ZOU
;
Shan WU
- Publication Type:Journal Article
- Keywords:
nuclear factor-kappa B;
intracerebral hemorrhage;
acute lung injury
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NF-?B in acute lung injury following intracerebral hemorrhage. Methods Using stereotactic apparatus we infused autologous blood into the caudate nucleus to build up rat ICH model. A total of 35 male adult Wistar rats were randomly divided into sham-operation group(n=5) and hemorrhage group(n=30) that was subsequently divided into 6 subgroups (6 h,12 h,24 h,48 h,3 d,and 5 d,n=5). The inflammatory reaction degrees in the peripheral tissues of hematoma in the brain and lung were observed after HE staining. The levels of NF-?B p65 mRNA in the brain and lung were assayed using RT-PCR. The content of TNF-? and IL-1? in serum was determined by radioimmunoassay (RIA) method. Results Brain edema was obvious at 12 h after intracerebral hemorrhage and the inflammatory cells infiltration increased at 24 h and reached maximum at 48 h in the peripheral tissues of hematoma. Interstitial pneumonia and local emphysema appeared at 6 h and got to peak at 48-72 h,in which alveolar structure was significantly destroyed. In comparison with sham-operation group,the expression of NF-?B p65 mRNA began to increase at 6 h in brain and lung tissues (P
