Analysis of Immunophenotype(I)、Morphology(M) and Cytogenetics(C) in 35 Cases of Chronic Lymphocytic Leukemia
- VernacularTitle:35例慢性淋巴细胞白血病MIC研究
- Author:
Qian LI
- Publication Type:Journal Article
- Keywords:
Chronic lymphocytic leukemia;
Morphology;
Immunophenotype;
Cytogenetics
- From:
Journal of Medical Research
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research immunophenotypic characteristics in CLL,and its correlation with the cytomorphology and cytogenetics. Methods Immunophenotyping was performed in 35 cases with CLL by flow cytometry analysis using a panel of monoclonal antibodies and CD45/SSC gating. Routine R-banding was performed and sequence specific probes for D13S272(13q14.3)、ATM(11q23) were used to detect del(13q14.3) and del(11q23) by FISH(fluorescence in situ hybridization ). Results Among 35 cases of CLL , 29 cases were B-lineage CLL, 4 cases were T-lineage CLL, 2 cases were CLL/PLL, 35 cases with CLL were CD5+, and among them B-CLL mainly were CD19+,CD20+ ,T-CLL mainly were CD2+,CD3+,CD7+,CD8+,2 CLL/PLL were CD5-.In morphology, in marrow or peripheral were mainly small mature lymphocytes,in CLL/PLL prolymphocytes exceed 11% and less than 50%. From 35 CLL,13 cases have cytogenetic abnormalities, 6 cases were number aberrations, concerning trisomy 3、12、14 and 18. 5 cases were structural abnormalities,2 cases were complex abnormalities. Seven cases with del(13q14.3)、del(11q23) were detected by two sequence specific probes for D13S272(13q14.3)、ATM(11q23). Conclusions The diagnosis of CLL was closely related with immunophenotyping 、morphology and cytogenetics.
