Clinical study on gut barrier dysfunction and bacterial translocation in patients following gastrointestinal surgery
- VernacularTitle:胃肠外科术后肠道细菌移位的临床研究
- Author:
Zhi QIAO
;
Rong LI
;
Zhanliang LI
- Publication Type:Journal Article
- Keywords:
surgical procedures, operative;
bacterial translocation;
pdymerase chain reaction;
lactic;
endotoxins
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate gut barrier dysfunction and bacterial translocation (BT) from gastrointestinal tract in postoperative patients using polymerase chain reaction (PCR) and plasma D-lactate measurement, and to study the relationship between BT and infections complications. Methods 34 patients undergoing selective gastrointestinal operations were observed. Blood samples were collected prior to and 2, 24, 48 h after surgery for bacterial culture, plasma D-lactate, and LPS measurement. PCR was used to detect microbial DNA. PCR was performed after DNA extraction, with target ?-lactosidase gene of E. coli and 16S rRNA gene of most pathogenic bacteria. All the patients were observed for 30 days for monitoring infectious complications. Results No bacterial DNA was detected before surgery, but it was positive in 7 patients (20.6%) after operation. Bacterial DNA was discovered in 38.9% (7/18) of SIRS (systemic inflammatory response syndrome) patients, no bacterial DNA was detected in the 16 non-SIRS patients (P
