Effect of hypoxia on NR1 subunit of NMDA receptor in rat cortex and hippocampus
- VernacularTitle:缺氧对大鼠皮层、海马NMDA受体NR1亚单位磷酸化的影响
- Author:
Xigui WU
;
Yandong ZHAO
;
Huaizhen RUAN
- Publication Type:Journal Article
- Keywords:
hypoxia;
cortex;
hippocampus;
NR1 subunit of N-methyl-D-aspartate receptor;
phosphorylation
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression and phosphorylation of N-methyl-D-aspartate (NMDA) receptor NR1 subunit in rat cortex and hippocampus under high altitude hypoxia. Methods The adult SD rats were exposed to hypobaric hypoxia imitating 5 500-meter high altitude for 8 h daily for 3, 7, 14, 21 d. Immunohistochemical staining, Western blotting and co-immunoprecipitation were used to detect the expression and phosphorylation of NR1 in rat cortex and hippocampus. Results Both immunohistochemistry and Western blotting showed NR1 expression in rat cortex and hippocampus was increased under hypoxia in a time-dependent manner. The tyrosine phosphorylation of hypoxia groups was increased, and reached the peak on day 14 after hypoxia, then decreased, still higher than that of control groups till day 21 (P
