The protective effects of polysaccharide from spirulina platensis on dopaminergic neurons of MPTP-induced Parkinsons disease model in mice
- VernacularTitle:螺旋藻多糖对MPTP致多巴胺能神经元损伤的保护
- Author:
Fang ZHANG
;
Junxia XIE
- Publication Type:Journal Article
- Keywords:
polysaccharide from spirulina platensis;
Parkinsons disease;
dopaminergic neurons;
mice
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The present study is designed to elucidate the protective effect of Polysaccharide from Spirulina Platensis(PSP) on dopaminergic neurons of MPTP-induced Parkinsons disease model in C57BL mice.Methods C57BL mice were divided into 5 groups:control group,MPTP group,PSP 200 mg?kg-1 plus MPTP group,PSP 400 mg?kg-1 plus MPTP group,and PSP 800 mg?kg-1 plus MPTP group.Immunohistochemistry assay,RT-PCR,and HPLC-ECD were used to detect the immunoreactivity of tyrosine hydroxylase(TH) and dopamine transporters(DAT),the expression of TH mRNA and DAT mRNA in substantia nigra(SN),the content of dopamine(DA) and its metabolites,i.e.3,4-dihydroxyphenyl acetic acid(DOPAC) and homovanilic acid(HVA) in striatum(Str).Results The result showed that,compared with control group,the TH and DAT immunoreactive stain in MPTP group was significantly decreased(P
