Effects of chloride channel blockers on H_2O_2 induced apoptosis in pancreas RIN-m beta cells
- VernacularTitle:氯通道阻断剂对H_2O_2诱导的胰岛RIN-m?细胞凋亡的影响
- Author:
Shenghua REN
;
Chunling YE
;
Yongliang JIN
;
Yanqing L
;
Juan FENG
- Publication Type:Journal Article
- Keywords:
pancreas RIN-m? cell,apoptosis,H_2O_2,chloride channel blocker
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of chloride channel blockers on the apoptosis of RIN-m? cells of pancreatic islet induced by H2O2.Methods The apoptotic model was made by H2O2 exposed for six hours with a concentration of 500 ?mol?L-1.The chloride channel blockers:DIDS,NPPB and NFA were administered to pretreat the samples respectively.The cell viability,morphological changes,and apoptosis rate were observed.Results Chloride channel blockers alone have no marked effects on the cell viability of RIN-m? cell.However,they elevated the cell viability of RIN-m?cell disposed of by H2O2.Compared to H2O2 group,the groups of DIDS +H2O2,NPPB+ H2O2 and NFA+H2O2 have significant difference in cell viability and apoptosis rate(P
