Effects of 2,3,4',5-tetrahydroxystilbene-2-O-?-D glucoside preventative administration on expression of nitric oxide synthase in atherosclerotic rats
- VernacularTitle:二苯乙烯苷预防性干预大鼠动脉硬化对其一氧化氮合酶表达的影响
- Author:
Wei ZHANG
;
Feng LI
;
Yuqin WANG
;
Chunhua WANG
;
Yan SHEN
- Publication Type:Journal Article
- Keywords:
tetrahydroxystilbene-glucoside;
atherosclerosis;
nitric oxide;
endothelium nitric oxide synthase;
inducible nitric oxide synthase
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of TSG on the content of nitric oxide and the expression of endo-thelium nitric oxide synthase in artery vessels of experimental atherosclerotic rats.Methods Sixty male rats were randomly divided into six groups.GroupⅠ,Normal control;GroupⅡ,Model control;Group Ⅲ,TSG low dose;Group Ⅳ,TSG middle dose;GroupⅤ,TSG high dose;group Ⅵ,Simvastatin.After 12 weeks,blood samples were collected from carotid arteries of rats,and the levels of NO in serum were measured.After that,the aortas were separated from the bodies and placed in-70℃,then the content of nitric oxide synthase was detected,and gene expression of eNOS and iNOSmRNA in artery vessels were respectively measured with RT-PCR method.STATA 8.0 software was adopted in date analysis..Results compared with those of the model group,the level of NO,the activity of NOS and the gene expression of eNOS were increased,and the gene expression of iNOS was reduced by simvastatin and TSG 30,60,120 mg?kg-1?d-1 in the high cholesterol-fed rats,which showed a dose-dependent effect.Conclusion TSG can enhance the expression of eNOS gene and reduce the expression of iNOS gene in aorta vessels of experimental atherosclerotic rats,which may be one of the anti-atherosclerosis mechanisms of TSG.
