Effects of GLU and GABA on the genesis of neurosteroids in primary cultured astrocytes
- VernacularTitle:谷氨酸和?-氨基丁酸对原代培养星形胶质细胞神经甾体合成释放的影响
- Author:
Yanning HOU
;
Ping JIANG
;
Honghai WU
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
glutamate;
?-aminobutyric acid;
astrocyte;
neurosteroid;
HPLC-MS
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate effects of glutamate(GLU) and ?-aminobutyric acid(GABA) on levels of neurosteroids synthesized and released by primary cultured rat brain cortical astrocytes.Methods Primary cultured rat brain cortical astrocytes were treated with GLU or GABA for 48 h respectively.Unconjugated(dehydroepiandrosterone,DHEA;pregnenolone,PREG;allopregnanolone,AP) and conjugated neurosteroids(dehydroepiandrosterone sulfate,DHEAS;pregnenolone sulfate,PREGS) in culture media were extracted by solid phase extraction(SPE) and analyzed by HPLC-MS.Results Compared with saline control group,GLU treatment decreased PREG and PREGS levels and increased DHEAS level significantly;GABA treatment decreased PREG level and increased AP level significantly.Conclusion Both GLU and GABA were shown to inhibit the genesis of PREG.While GLU significantly promoted the genesis of DHEAS and GABA promoted the genesis of AP.The genesis of PREGS was also inhibited by GLU treatment in primary cultured cortical astrocytes.
