Establishment of a "Four-step method" for extraction and separation of Candida albicans total proteins
- VernacularTitle:一种四步法提取分离白念珠菌总蛋白质方法的建立
- Author:
Yi XU
;
Lan YAN
;
Jundong ZHANG
;
Yuanying JIANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
proteome;
four-step method;
electrophoresis,gel,two-dimensional
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To introduce a "Four-step method" for extraction and separation of Candida albicans total proteins.Methods: Proteins of C.albicans were extracted step-by-step with 4 kinds of solutions with different solubilities.After quantification,the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.Results: Proteins with different solubilities were successfully extracted step-by-step from C.albicans and were separated by two-dimensional gel electrophoresis. Conclusion: The "Four-step method" for extraction of C.albicans proteins is an effective approach to study C.albicans membrane proteome and lays a foundation for further investigation of the mechanisms of antifungal agents and drug resistance in C.albicans.
