Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells
- VernacularTitle:单核细胞趋化蛋白-1对人脐静脉内皮细胞凋亡的影响
- Author:
Qinshan LI
;
Yang LIU
;
Zanjie FENG
;
Yaling ZHANG
;
Minzhang QIAN
- Publication Type:Journal Article
- Keywords:
monocyte chemotactic protein-1;
human umbilical vein endothelial cell;
apoptosis;
atherosclerosis
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of monocyte chemotactic protein-1(MCP-1) on the growth of human umbilical vein endothelial cells (hUVEC). Methods hUVEC were cultured in vitro and identified. hUVEC that grew vigorously were stimulated for 24 h or 48 h respectively with MCP-1(0.1, 1.0, 10, and 100 ?g/L). The survival rate of hUVEC was first detected by MTT assay. The cell cycle and DNA content were detected and analyzed by flow cytometry. Results hUVEC were isolated from human umbilical veins and cultured, and then identified by immunofluorescence and immunohistochemistry with factor Ⅷ and KDR. MCP-1 induced the apoptosis of hUVEC in a dose-dependentmanner and a time-dependentmanner (P
