Expression and location of IL-2/IFN-? fusion protein in colorectal tumor cell lines
- VernacularTitle:结直肠癌细胞中白细胞介素-2和干扰素-?融合蛋白的表达及定位
- Author:
Yan WANG
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Carcinoembryonic antigen,promotor;
Cytokine gene therapy;
Fusion gene
- From:
Chinese Journal of Practical Internal Medicine
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.
