Characterization of a novel full-length gene associated with human pancreatic cancer
- VernacularTitle:一条全长胰腺癌相关新基因的初步研究
- Author:
Yegui JIANG
;
Zhaoshen LI
;
Tonghua LIU
;
Yuming WANG
- Publication Type:Journal Article
- Keywords:
pancreatic cancer;
cDNA microarray;
gene expression spectrum
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify a novel full-length human pancreatic cancer-associated gene screened by cDNA microarray. Methods One novel full-length gene which overexpressed in the pancreatic cancer was sequenced and analyzed for bioinformatics. The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreatic tissues and 4 pancreatic cancer cell lines were detected by RT-PCR and Northern blot analysis. Results Bioinformatics analysis showed that the chromatosome of the novel gene localized to 4p15, and had an open frame of 501 base pair encoding 166 amino acids of protein. Its theoretical molecular weight was 18 293.23 and PI was 8.57. This protein had more than 90% homologous human hypothetical protein FLJ90013 using BLASTp analysis, and may be a new member of FLJ90013 protein family. The novel gene expressed in 12 specimens of pancreatic cancer tissues and 4 pancreatic cancer cell lines, but not in normal pancreatic tissues. This result was just the same as the result of Northern blot analysis. Conclusion The analysis of gene expression profile of tumor based on cDNA microarray can realize high-throughput screening of the genes associated with the pancreatic cancer, and it can help to rapidly explore the gene function. The novel gene is associated with oncogenesis and development of pancreatic cancer, and might be potential tumor marker or molecular target for diagnosis and treatment of pancreatic cancer.
