Effects of EGF on Isolation and Cloning of Mouse Embryonic Stem-like Cells from PGCs
- VernacularTitle:EGF对原始生殖细胞来源小鼠胚胎干细胞分离克隆的影响
- Author:
Song LI
;
Zhongying DOU
- Publication Type:Journal Article
- Keywords:
Cytokines;
Mouse embryonic stem-like cells;
Isolation and cloning
- From:
Journal of Medical Research
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the EGF on isolation and cloning of mouse embryonic stem-like cells from PGCs. Methods EGF with different density were added to the culture medium of cells .After a culturing period ,the cloning number of mouse embryonic stem-like cells and the topmost passage would be observed and registered. Results There was not obvious effects on the primary culture of mouse ESC and was distinct on the later passage if EGF were used. Conclusions In the primary passage of mouse ESC may survive without EGF,and the impact of the existence of EGF was better than the one of the inexistence to the cloning of mouse ESC.
