The adhesive characters of Lactobacillus to enterocyte-like HT-29 cells
- VernacularTitle:乳杆菌对肠上皮样细胞HT-29的黏附特性
- Author:
Bin WANG
;
Hong WEI
;
Qiurong LI
- Publication Type:Journal Article
- Keywords:
Lactobacillus;
bacterial adhesion;
HT-29 cells
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the adhesive characters of Lactobacillus to enterocyte-like HT-29 cells.Methods A HT-29 cell culture was employed to investigate the adherent ability of Lactobacillus.Bacterial suspension and HT-29 cells were co-incubated at 37℃ for 1h.The cells with adherent bacteria were Gram-stained,and then examined microscopically under oil immersion.The pH of the medium,bacterial growth phase,co-incubation time,bacterial concentration,D-mannose and type of host cells,which were involved in the process of adherence of Lactobacillus to enterocyte-like HT-29 cells,were also investigated.Results The adherent level increased substantially for Lactobacillus reuteri JCM1081 as the pH decreased from 6.0 to 4.0.Maximum adherence occurred within 90min and remained stable until the end of incubation.The bacterial concentration exerted a certain influence on the adherence of Lactobacillus reuteri JCM1081 to HT-29 cells,and it seemed to be concentration dependent.Maximum adherence occurred in the concentration of 109cfu/ml and remained stable until the end of incubation.Lactobacillus strain that grew into stationary phase showed higher ability to adhere to HT-29 cells than that in logarithmic phase.The addition of D-mannose dramatically decreased the level of Lactobacillus adherence.The Lactobacillus strain adhered in higher levels to the enterocyte-like cells or gastric epithelium cells than other cell origins,and the adhesion of Lactobacillus showed host cells specificity.Conclusion The adhesive characters observed with Lactobacillus suggested that adhesive properties are species and host specific.The pH,bacterial growth phase,co-incubation time,bacterial concentration and D-mannose may influence the adhesion of Lactobacillus to enterocyte-like HT-29 cells.
