Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV_3
- VernacularTitle:genistein抑制人卵巢浆液性乳头状囊腺癌细胞系SKOV_3体外侵袭作用的分子机制
- Author:
Yu LI
;
Can MI
- Publication Type:Journal Article
- Keywords:
genistein;
ovarian tumor;
invasion
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how genistein to inhibit the invasion of human ovarian serous papillary cystadenocarcinoma cell line SKOV3.Methods Millicell chamber and coculture method were used to establish chemotactic migration model in vitro to observe the effect of genistein on directional chemotactic migration movement of SKOV3 cells.The protein expressions of cell surface adhesion molecule CD44v6,matrix metalloproteinases and tissue inhibitor of metalloproteinases MMP-9/TIMP-1 and MMP-2/TIMP-2 were determined by using immunocytochemical method and their mRNA levels were examined by in situ hybridization.Results Compared with control group,the directional chemotactic migration of SKOV3 were significantly decreased after treated with 20 ?mol/L and 40 ?mol/L genistein,reached to(46.9?5.8)% and(28.3?4.7)% respectively(P
