Effect of ginaton on renal tubular epithelial cell apoptosis induced by renal ischemia/reperfusion
- VernacularTitle:金纳多对肾缺血/再灌注诱导的肾小管上皮细胞凋亡的影响
- Author:
Yan WANG
;
Huaixue JI
;
Shuhua XING
- Publication Type:Journal Article
- Keywords:
JNK;
c-Jun;
Ginkgo biloba extract;
renal ischemia/reperfusion;
cell apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of ginaton on tubular cell apoptosis in a rat model of renal ischemia/reperfusion(I/R) injury.Methods The model of bilateral post-ischemic renal injury was established by clamping renal pedicles for 45 minutes.To explore the effects of ginaton on activation and expression of JNK and c-Jun,p-JNK1/2,JNK,p-c-Jun,c-Jun were examined by Western blotting.Apoptosis of renal tubular epithelial cells was assessed by terminal deoxynucleotidyl transferase mediated dUTPnick end labeling(TUNEL) and flow cytometric analysis.Results Phosphorylation levels of the JNK and the c-Jun were significantly increased at 20 min after renal I/R injury.This corresponds to a significant increase of apoptotic tubular cells induced by renal I/R injury.Ginaton attenuated the increased phosphorylation of p-JUK at 20 min(vs I/R 20 min,P
