The inhibitive effect of Cariporide in LPC-induced adhesion of monocytes and endothelial cells by suppressing expression of ICAM-1 and P-selectin in vitro
- VernacularTitle:Cariporide对LPC诱导单核细胞与内皮细胞粘附及粘附分子表达的抑制作用
- Author:
Zhi LI
;
Liying LIU
- Publication Type:Journal Article
- Keywords:
Na~+/H~+ exchanger;
pH;
lysophosphatidylcholine;
Cariporide;
adhesion molecule
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Cariporide on expression of intercellular adhesion molecule-1(ICAM-1) and P-selectin and adhesion of monocytes and endothelial cells induced by lysophophatidylcholine(LPC).Methods The ratio of adhesion of monocytes and endothelial cells was assessed by measuring protein content.The expression of ICAM-1 and P-selectin in endothelial cells was examined by enzyme-linked immunosorbent assay(ELISA).Intracellular pH of endothelial cells was measured with 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein(BCECF).Results The results showed that LPC enhanced the adhesion of monocytes and endothelial cells in a concentration-dependant and time-related manner.The dosage of 5,10 and 20 ?mol?L-1 Cariporide reduced the adhesive ratio of endothelial cells induced by LPC(5 mg?L-1)from 36% to 23%,18% and 16%(P
