Effects of high density lipoprotein and apolipoprotein A-Ⅰ mimetic peptide on the expression of adiponectin in 3T3-L1 adipocytes
- VernacularTitle:HDL和ApoA-Ⅰ模拟肽对3T3-L1脂肪细胞脂联素表达的影响
- Author:
Xiangzhu XIE
;
Shuiping ZHAO
;
Bilian YU
;
Qiaoqing ZHONG
- Publication Type:Journal Article
- Keywords:
high density lipoprotein;
apolipoprotein A-I mimetic peptide;
adipocytes;
adiponectin;
peroxisome proliferator-activated receptor gamma;
nuclear factor-kappa B
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of high density lipoprotein and apolipoprotein A-I mimetic peptide on the secretion and the expression of adiponectin in fully differentiated 3T3-L1 adipocytes in inflammatory status and the possible mechanism.Methods Fully differentiated 3T3-L1 adipocytes were incubated in the medium containing various concentrations of high density lipoprotein(10~100 mg?L-1) and apolipoprotein A-I mimetic peptide(1~50 mg?L-1)with 100 ?g?L-1 lipopolysaccharide(n=3).Evaluate the levels of adiponectin in supernatant,the mRNA expression of adiponectin and PPAR?,and the activity of NF-?B.Results During LPS stimulation adiponectin concentrations in supernatant decreased from 0.25?0.03 ?g?L-1 to 0.14?0.02 ?g?L-1(P
