Oxidized low density lipoprotein-induced expression of transforming growth factor beta 1 and production of extracellular matrix in rat glomerular mesangial cells is mediated by lectin-like oxidized low density lipoprotein receptor-1
- VernacularTitle:LOX-1介导ox-LDL诱导大鼠系膜细胞表达TGF-?_1及分泌细胞外基质
- Author:
Yueguang DU
- Publication Type:Journal Article
- Keywords:
diabetic nephropathies;
glomerulosclerosis;
oxidized low density lipoprotein;
lectin-like oxidized low density lipoprotein receptor 1;
transforming growth factor beta 1;
extracellular matrix
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of oxidized low-density lipoprotein(ox-LDL)on expression of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1),transforming growth factor beta 1(TGF-?_1),and secretion of extracelluar matrix(ECM)in cultured rat glomerular mesangial cells(GMCs),and to investigate the influence of LOX-1 inhibitor polyinosinic acid(PIA)on the effect of ox-LDL.Methods:Rat glomerular masengial cells were cultured in vitro.RT-PCR was employed to determine the LOX-1 mRNA expression in GMCs incubated with different concentrations of ox-LDL(0,25,50, 100?g/ml).The expression of LOX-1 and TGF-?_1 mRNA was also determined by RT-PCR in the blank control group,ox-LDL (50?g/ml)group and PIA(50?g/ml ox-LDL+250?g/ml PIA)group.The contents of TGF-?_1,fibronectin(FN),and collagenⅣ(ColⅣ)in the supernatants of the above 3 groups were determined by ELISA.Results:RT-PCR showed that LOX-1 mRNA expression in 25,50 and 100?g/ml ox-LDL groups was significantly higher than that of blank control group(P
