Human lung fibroblasts exhibit differentiation potential as bone marrow mesenchymal stem cells
- VernacularTitle:人肺成纤维母细胞表达骨髓间充质干细胞特性的研究
- Author:
Qiuhong FANG
;
Chaoxiang SHUI
;
Yaoyao WANG
- Publication Type:Journal Article
- Keywords:
Lung fibroblast;
Osteoblast;
Adipocyte;
Bone marrow mesenchymal stem cell
- From:
Chinese Journal of Practical Internal Medicine
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differentiation potential of human lung fibroblasts.Methods The study was performed in First Hospital of Tsinghua University from March 2006 to October 2006.Human lung fibroblasts were cultured for 2 weeks in an osteogenic medium[containing 10% fetal calf serum (FCS)and 50 g/mL Vitamin C and 10 mmol/L ?-glycerophospate],adipogenic medium(containing 15% horse serum,10-8 mol/L dexmethasone and 10 mg/L insulin),or control medium(10% FCS).Osteoblasts were detected by alkaline phosphatese and calcium salt staining.The expression of osteopontin was measured by Western blotting,Oil Red -O staining for identification of mature adipocytes.Results With the induction of osteogenic medium for 2 weeks,the expression of alkaline phosphatese and osteopontin was increased and the deposition of calcium salt was detected.Mature adipocytes formed after culture with adipogenic medium for 2 weeks.Conclusion Under certain condition,human lung fibroblasts can differentiate into osteoblasts or adipocytes,which were characterized by bone marrow mesenchymal stem cells.
