INFLUENCE OF DIETARY RESTRICTION ON THE EXPRESSION OF INSULIN SIGNALING GENES IN MOUSE EPIDIDYMIS FAT PAD
- VernacularTitle:限食对小鼠附睾脂肪垫胰岛素信号通路基因表达的影响
- Author:
Hao WU
;
Hongwei GUO
- Publication Type:Journal Article
- Keywords:
mice;
dietary restriction;
insulin signaling pathway gene
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of dietary restriction on the expression of insulin signaling pathway genes in mouse adipose tissue. Method:Twenty four C57BL/6 male mice of 2 w age were subjected to either AL (ad libitum) or DR (dietary restriction, 40% from AL) randomly until sacrificed.Then the epididymis fat pads were weighed, and their insulin signaling gene expression was detected by Oligo Insulin Signaling Pathway Microarray (SuperArray), and two of the differentially expressed genes, NPY and AKT3 were validated by Real Time PCR. Results:Dietary restriction significantly reduced the weight of epididymis fat pads and 31 (20 up, 11 down regulation) of 96 insulin signaling pathway genes were differentially expressed. The results of Microarray coincided with that of Real Time PCR. Conclusion:The differential regulation on the expressions of insulin signaling pathway gene may be the important molecular basis of dietary restriction.
