EFFECT OF OSTEOPROTEGERIN GENE POLYMORPHISM ON BONE MASS IN PREMENARCHE GIRLS
- VernacularTitle:护骨素基因多态性对青春前期女童骨量的影响
- Publication Type:Journal Article
- Keywords: OPG; PCR-RFLP; polymorphism; bone mineral density
- From: Acta Nutrimenta Sinica 1956;0(04):-
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the effects of promoter region A163G,T245G and T950C polymorphisms of osteoprotegerin (OPG) gene on bone mineral density (BMD) in premenarche girls. Method: The OPG genotypes were determined by PCR-RFLP in 214 premenarche girls of Han nationality in Guangzhou area , and BMC and BMD at lumbar spine(L1-4), femoral neck, Ward’s triangle area and total BMD were measured by dual-energy X-ray absorptiometry(DEXA). Three markers of bone turnover were also detected. Results: Hardy-Weinberg equilibrium was evident for OPG polymorphisms. The frequency of three genotypes were as following:A163G:AA-74.8%,AG-21.5%,GG-3.7%;T245G:TT-77.1%, TG-21.5%,GG-1.4%;T950C:TT-41.1%,TC-50.5%,CC-8.4%. The girls with CC genotype had higher BMD at femoral neck , Ward’s triangle area and total BMD than those with TC or TT genotypes. Such difference was not detected between girls with TC genotype and those with TT genotype. Significant link was not observed between A163G、T245G polymorphism with BMD. Conclusion: The premenarche girls with T allele may have lower BMD at femoral neck , Ward’s triangle area and total BMD. The results mayprovide scientific basis for further determining a valuable genetic marker for low bone mass in premenarche girls.