Relationship of IL-6/STAT3 with liver regeneration of partial grafts after cold preservation in rats
- VernacularTitle:IL-6/STAT3与冷保存大鼠部分肝移植肝再生的关系
- Author:
Shengguang YUAN
;
Jijian WANG
;
Jiahong DONG
- Publication Type:Journal Article
- Keywords:
IL-6;
STAT3;
liver regeneration;
liver transplantation;
rats
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of different cold preservation time on liver regeneration after partial liver transplantation in rats,and study the role of IL-6/STAT3.Methods SD rats were divided into 3 groups:group Ⅰ underwent liver resection,group Ⅱ received partial liver transplantation after one-hour cold preservation and group Ⅲ received partial liver transplantation after 8-hour cold preservation.Radioimmunoassay was used to measure the levels of TNF-? and IL-6 in liver tissues.The protein expressions of PCNA and STAT3 were assessed by immunohistochemical staining in paraffin sections.The liver regeneration ratio and the expressions of the proliferative cell nuclear antigen(PCNA)and STAT3 were compared among three groups at 1,6,12,24,48,72 and 168 h after operation.Results The remnant liver in group Ⅰ and grafts in group Ⅱ grew rapidly;The regeneration ratio in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ during postoperative day 2 to 3(P
