To Explore the Double-labeling Method of Monitoring the GHRP Regulatory Function on [Ca~(2+)]i and NO on Real Time in Cardiomyocytes Under LSCM
- VernacularTitle:实时监测GHRP对心肌细胞内[Ca~(2+)]i和NO调控的双标记方法研究
- Author:
Meixiu LI
;
Yanru WANG
;
Jimin CAO
;
Guozhong TIAN
;
Linghui ZHAO
- Publication Type:Journal Article
- Keywords:
Cardiomyocyte;
The concentration of intracellular free Ca2+([Ca2+]i);
Nitric oxide(NO);
Double-label:Growth hormone-releasing peptide(GHRP);
Laser scanning confocal microscope(LSCM)
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the double-labeling method of monitoring the GHRP regulatory function on [Ca2+]i and NO in cardiomyocytes of rats on real time under LSCM.Methods The reformed constant-flow Langendorff system and enzyme-dissociated was used to isolate cardiomyocytes.[Ca2+]i and NO in the cardiomyocytes of SD rats were double-labeled by their molecular probe Rhod-2/AM and DAF-FM/DA,respectively to monitor the regulatory function of GHRP on [Ca2+]i and NO on real time by LSCM.Results Ca2+ signal showed a red fluorescence and NO showed a green fluorescence while the overlapping of the two signals showed a yellow-green fluorescence by this system,and the similar effect presents in both double-labeled state and the single labeled one:GHRP induced a transient[Ca2+]i increase then followed by a plateau phase while there was not significant change in NO signal system after GHRP stimulation under the LSCM in the cardiomyocytes of rats.Conclusions After having established the double-labeling method we monitored the GHRP regulatory function on [Ca2+]i and NO on real time in cardiomyocytes of rats under LSCM causing the [Ca2+]i biphasic increase while no significant change in NO signal system.
