Effects of rhG-CSF mobilization on immunological characteristics of peripheral blood grafts and steady-state bone marrow
- VernacularTitle:人重组粒细胞集落刺激因子动员的外周血采集物和稳态骨髓采集物免疫学特性的对比研究
- Author:
Yingjun CHANG
;
Xiangyu ZHAO
;
Xiaojun HUANG
- Publication Type:Journal Article
- Keywords:
Granulocyte colony-stimulating factor;
Peripheral blood stem cell grafts;
Steady state bone marrow grafts;
T cell hyporesponsiveness;
Dendritic cells;
Costimulatory molecules
- From:
Chinese Journal of Practical Internal Medicine
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the difference of immunological characteristics between recombination human granulocyte colony-stimulating factor(rhG-CSF)mobilized peripheral blood grafts(G-PB)and steady-state bone marrow grafts(SS-BM).Methods From April to October 2003,G-PB and SS-BM of 15 related donors were collected.T cell subgroups,dendritic cells(DC),monocytes and the expression of CD28 costimulatory molecules on T cells were determined by multicolor flow cytometry.The lymphocyte proliferation ability and the quantities of interleukin-4(IL-4)and interferon-?(IFN-?)secreted by T cells were determined using MTT assays and sandwich ELISA.Results The absolute numbers of monocytes,CD3+,CD4+ and CD8+ T cells,and the ratios of CD4/CD8 in G-PB were significantly higher than those in SS-BM,respectively(P0.05).The quantities of IFN-? and IL-4 secreted by T cells per micromilter in G-PB was significantly higher than those in SS-BM(P0.05).The absolute numbers of DC1 and DC2 in G-PB were significantly higher than those in SS-BM(P0.05).Conclusion It is concluded that the difference of immunological characteristics between G-PB and SS-BM may explain the lower incidence of GVHD and lower relapse rate after SS-BM and G-PB transplantation.
