Pathologic and VEGF changes of transplantation of endothelial progenitor cells for hindlimb ischemia in diabetic rabbit model
- VernacularTitle:内皮祖细胞移植对糖尿病兔下肢缺血的病理及血管内皮生长因子的影响
- Author:
Ping YU
;
Qiang LI
;
Ying LIU
- Publication Type:Journal Article
- Keywords:
EPC;
Transplantation;
Diabetic rabbit model;
Hindlimb ischemia
- From:
Chinese Journal of Practical Internal Medicine
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of transplantation of endothelial progenitor cells(EPC)from autologous bone marrow for hindlimb ischemia in diabetic rabbit model,and the difference between diabetes and nondiabetes.Methods Rabbits were randomly allocated into three groups for transplantation therapy,PBS control group(n=8,A group),EPC transplantation with diabetes group(n=14,B group),EPC transplantation without diabetes group(n=8,C group).Diabetic rabbit model was established by injecting alloxan,and hindlimb ischemia was induced by complete excision of femoral artery.Diabetic rabbits with hindlimb ischemia were therapied by transplanted ex vivo expanded EPC,and then effects were assessed by capillary density and capillary to muscle fiber ratio,and VEGF in muscle was also determined.Results Capillary density,capillary to muscle fiber ratio,and VEGF in muscle increased significantly in EPC transplantation group with or without diabetes after transplanting 14 days(P
