Condensation mechanism of aminoglucomannan with plasmid DNA
- VernacularTitle:氨基甘露聚糖对质粒DNA构象的影响因素及机制研究
- Author:
Yuan GUO
;
Yongkang LIU
;
Hongtao LI
;
Ying DU
;
Guomin CHEN
- Publication Type:Journal Article
- Keywords:
aminoglucomannan;
plasmid DNA;
condensation
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the condensation mechanism of aminoglucomannan(AGM)with plasmid DNA and some influencing factors in this process.Methods The polysaccharide-DNA complex was analyzed with agarose gel electrophoresis by observing the changes of DNA bands under various influencing conditions including different pH,ionic strength(NaCl concentration),temperature and plasmid DNA sorts.Results The new bands appeared in the slow migration positions after reaction between AGM and plasmid DNA,and the changes of DNA bands content were dependent on AGM concentrations.The condensation degree could be effected by pH,temperature,and ionic strength.Conclusion Our study suggests that the AGM can be condensed with plasmid DNA in proper conditions.
