Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells
- VernacularTitle:FGFR3转染小鼠骨髓间充质干细胞对CbfaⅠ和CollagenⅠ表达的影响
- Author:
Zhijun LIU
;
Lin CHEN
- Publication Type:Journal Article
- Keywords:
fibroblast growth factor receptor 3;
mesenchymal stem cells;
transfection
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of fibroblast growth factor receptor 3(FGFR3)on the expressions of CbfaⅠ and Collagen Ⅰ in mesenchymal stem cells(MSCs)after Ad-FGFR3 transfection.Methods After Ad-FGFR3 and Ad-GFP were respectively transfected into mouse mesenchymal stem cells,the expression of FGFR3,CbfaⅠ and Collagen Ⅰ mRNAs and proteins were detected by RT-PCR and Western blot.Results The expressions of FGFR3,CbfaⅠ and Collagen Ⅰ were significantly higher in Ad-FGFR3 transfected MSCs than in Ad-GFP group.Conclusion FGFR3 can up-regulate the expression of CbfaⅠ and Collagen Ⅰ.
