Primary studies on the anti-uterine cervix cancer effects of the extract ZE4 from Lysmachia clethroides Duby
- VernacularTitle:珍珠菜提取物ZE4对宫颈癌抑制作用的初步研究
- Author:
Yiqian WANG
;
Lihua TANG
;
Zhongqin LIANG
;
Zhenlun GU
- Publication Type:Journal Article
- Keywords:
Lysmachia Clethroides Duby;
uterine cervix cancer;
3H-TdR;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To estimate the anti-uterine cervix cancer effects of the extracts from Lysimachia clethroides Duby in vivo and in vitro. Methods Inhibitory effect on growth was observed by MTT, 3H-TdR and colony-forming units assay. Apoptosis was detected by Hoechst fluorescent staining analysis. The effect on cell migration and morphology was observed by scratch test; Detecting effects of ZE4 on transplant tumor (U14) in mice through observing the tumor weight and organ index. Results ZE4 could inhibit the growth and migration of HeLa cell significantly and induce cell apoptosis. Its half inhibitory concentration (IC50) at 48h was 40.56 mg?L-1 by 3H-TdR assay. The inhibition rate of ZE4 against U14 was 49.9% at the dose of 400 mg?kg-1. Conclusions ZE4 could inhibit the growth of uterine cervix cancer in vitro and in vivo.
