Changes of HO/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition and treatment with Renshen,Shuangshen,and Tongxinluo
- VernacularTitle:络气虚滞大鼠动脉组织血红素加氧酶/一氧化碳系统的变化及人参、双参、通心络的干预
- Author:
Junqing LIANG
;
Yiling WU
;
Zhenhua JIA
;
Haibo XU
;
Chunhua DING
;
Hongtao WANG
- Publication Type:Journal Article
- Keywords:
stagnancy of collateral-Qi in deficiency condition;
heme oxygenase;
carbon monoxide;
herbs to dredge collaterals
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the vascular protective mechanism of different levels of herbs through detecting activity of HO-1/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition.Methods:Healthy male Wistar rats of cleanness level were randomly divided into control group,stagnancy of collateral-Qi in a deficiency condition group(SCQDC group),Renshen group,Shuangshen group,and Tongxinluo group.The ultrastructure of endothelium cells in the artery was observed.RT-PCR,Western blot,and immunohistochemical approaches were used to detect JNK,c-Jun mRNA and albumen expression in the arterial tissues.Meanwhile,the content of CO in the arterial tissue was also detected.Results:In SCQDC group,the counts of pinocytotic cells and microvili on the free surface of vascular endothelial cells were significantly decreased;most crests of mitochondria fused with membrane,some even disappeared;serious degranulation was observed in the rough surfaced endoplasmic reticulum.Immunohistochemistry showed that the HO-1 positive signals in the arterial histiocyte weakened significantly compared with the control group.The HO-1 expression was increased in the 3 treatment groups.Compared with control group,SCQDC group had decreased HO-1 mRNA and albumen expression(P
