Inhibition of sinomenine on nuclear factor-?B of synoviocytes in collagen-induced arthritis rats
- VernacularTitle:青藤碱对胶原诱导性关节炎大鼠滑膜细胞NF-?B的抑制作用
- Author:
Xiaolu HUANG
;
Fei HAO
;
Yong WANG
;
Yongfei FANG
- Publication Type:Journal Article
- Keywords:
sinomenine;
collagen-induced arthritis;
synoviocytes;
nuclear factors-?B;
nuclear translocation
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of sinomenine(SN) in vitro on nuclear factor-?B(NF-?B) DNA binding activity and nuclear translocation of synoviocytes in collagen-induced arthritis(CIA) rats and explore its antiinflammatory mechanisms.Methods The experimental model of CIA rats was used and synoviocytes were collected.Cells were divided into five groups:normal control,CIA,CIA+10 ?mol/L methotrexate(MTX),CIA+50 ?mol/L SN,CIA+500 ?mol/L SN.Nuclear translocation of NF-?B p65 subunit and NF-?B DNA binding activity of synoviocytes were investigated by fluorescence labelling laser confocal scanning microscopy and electrophoretic mobility shift assay(EMSA) respectively.Results Compared to normal control,significant nuclear translocation of NF-?B p65 subunit was observed and NF-?B DNA binding activity was increased in synoviocytes of CIA rats(P
