Effect of Lactobacillus plantarun onlly regulating intestinal epithelial permeability and tight junction response to pathogenic bacteria
- VernacularTitle:乳酸菌对感染肠上皮细胞通透性及紧密连接蛋白表达的影响
- Author:
Zhongwei ZHANG
;
Huanlong QIN
- Publication Type:Journal Article
- Keywords:
Lactobacillus;
Tight junction;
Claudin-1;
Occludin
- From:
Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Abjective:To investigate the effect of Lactobacillus plantarun Onlly regulating the intestine epithelial permeability and tight junction proteins of enterocyte-like Caco-2 cells in responsing to Enteroinvasive Escherichia coli.Methods:In the model of Caco-2 monolayer cells,The cells were divided into four groups: normal group,infection group,Lactobacillus group and gentamicin group.The TER was measured by Milicell ERS and the transmissivity of mannitol was determined by HPLC.The distribution and structure of tight junction proteins,such as Claudin-1,Occludin,ZO-1,JAM-1 were examined by indirect IHC.Results: The TER and the transmissivity of mannitol increased after infected with EIEC,but the increase was decreased after dealing with Lactobacillus plantarun Onlly(P
